Hygiene of populated placesISSN: 2707-0441 eISSN: 2707-045X
Issue 73, 2023   -   Pages: 59-67
PROCESSES OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN BLOOD AND LIVER UNDER LEAD EXPOSURE AND THEIR CORRECTION IN EXPERIMENTAL STUDIES
Yu.V. Fedorenko1
1 DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY

: 612.015.11:615.9:546.81]-08
https://doi.org/10.32402/hygiene2023.73.059

SUMMRY:
Objective. To investigate the changes over time in the indicators of lipid peroxidation and antioxidant protection in blood and liver tissue under lead exposure before and during biological correction.
Materials and methods. Experimental studies have been conducted on white male rats weighing 170-200 g for 30 days. Animals were divided into two groups: the first group received Pb(NO3)2, and the second group received both Pb(NO3)2 and bioprotectors. Aqueous solution of lead nitrate was injected into the stomach fasting at a dose of 36 mg/kg of body weight. Pectin, calcium, and antioxidants C, E, β-carotene vitamins and selenium (Triovit capsule) were used as biological protectors against the negative effects of lead. Drinking water was administered to the animals of the control groups, and appropriately selected bioprotectors were added to the diet. The conjugated dienes, malondialdehyde, superoxide dismutase activity, catalase activity, and antioxidant activity index were determined in the blood and liver tissue. An integral coefficient characterizing the antioxidant state of the body was calculated based on the ratios of the antioxidant protection indicator activity and the intensity of lipid peroxidation processes.
Results. It was found that during the experiment the level of lipid peroxidation products was increased: conjugated dienes by 70.8-114.8% in the blood, by 27.3-94.6% in the liver, malondialdehyde by 28.3-97.6% and 24.5-91.5%, respectively, compared to the controls. At the same time, on day 15 of the experiment, the activity of superoxide dismutase increased by 34.2% in the blood, the enzyme activity practically did not change in the liver tissue, and the catalase activity decreased. At the end of the experiment, the activity of superoxide dismutase and catalase enzymes decreased, to a greater extent in blood than in liver tissue. The obtained results indicate an increase in oxidative stress in the dynamics of lead intoxication, which is confirmed by low values of integral coefficients. The addition of bioprotector complex to the diet throughout the experiment with the simultaneous lead intake restores balance in the system of lipid peroxidation antioxidant protection of liver and blood tissue.
Conclusions. Lead nitrate has induced oxidative stress in experimental animals. The complex of bioprotectors pectin, calcium, C, E, and β-carotene vitamins, and selenium, activates metabolic processes and optimally corrects the disturbance in the activity ratio of processes in the pro- and antioxidant system caused by the action of lead.

KEYWORDS:
Lead nitrate, blood, liver tissue, conjugated dienes, malondialdehyde, superoxide dismutase, catalase, bioprotectors.

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FOR CITATION:
Fedorenko YuV. [Processes of lipid peroxidation and antioxidant protection in blood and liver under lead exposure and their correction in experimental studies]. Hygiene of Populated Places. 2023;73:59-67. Ukrainian.