| Hygiene of populated places | ISSN: 2707-0441 eISSN: 2707-045X |
Issue 73, 2023 - Pages: 59-67
PROCESSES OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN BLOOD AND LIVER UNDER LEAD EXPOSURE AND THEIR CORRECTION IN EXPERIMENTAL STUDIES
Yu.V. Fedorenko1
1 DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
ÓÄÊ: 612.015.11:615.9:546.81]-08
SUMMÀRY:
Objective. To investigate the changes over time in the indicators of lipid peroxidation and antioxidant protection in blood and liver tissue under lead exposure before and during biological correction.
Materials and methods. Experimental studies have been conducted on white male rats weighing 170-200 g for 30 days. Animals were divided into two groups: the first group received Pb(NO3)2, and the second group received both Pb(NO3)2 and bioprotectors. Aqueous solution of lead nitrate was injected into the stomach fasting at a dose of 36 mg/kg of body weight. Pectin, calcium, and antioxidants – C, E, β-carotene vitamins and selenium (Triovit® capsule) were used as biological protectors against the negative effects of lead. Drinking water was administered to the animals of the control groups, and appropriately selected bioprotectors were added to the diet. The conjugated dienes, malondialdehyde, superoxide dismutase activity, catalase activity, and antioxidant activity index were determined in the blood and liver tissue. An integral coefficient characterizing the antioxidant state of the body was calculated based on the ratios of the antioxidant protection indicator activity and the intensity of lipid peroxidation processes.
Results. It was found that during the experiment the level of lipid peroxidation products was increased: conjugated dienes by 70.8-114.8% in the blood, by 27.3-94.6% in the liver, malondialdehyde by 28.3-97.6% and 24.5-91.5%, respectively, compared to the controls. At the same time, on day 15 of the experiment, the activity of superoxide dismutase increased by 34.2% in the blood, the enzyme activity practically did not change in the liver tissue, and the catalase activity decreased. At the end of the experiment, the activity of superoxide dismutase and catalase enzymes decreased, to a greater extent in blood than in liver tissue. The obtained results indicate an increase in oxidative stress in the dynamics of lead intoxication, which is confirmed by low values of integral coefficients. The addition of bioprotector complex to the diet throughout the experiment with the simultaneous lead intake restores balance in the system of lipid peroxidation – antioxidant protection of liver and blood tissue.
Conclusions. Lead nitrate has induced oxidative stress in experimental animals. The complex of bioprotectors – pectin, calcium, C, E, and β-carotene vitamins, and selenium, activates metabolic processes and optimally corrects the disturbance in the activity ratio of processes in the pro- and antioxidant system caused by the action of lead.
Materials and methods. Experimental studies have been conducted on white male rats weighing 170-200 g for 30 days. Animals were divided into two groups: the first group received Pb(NO3)2, and the second group received both Pb(NO3)2 and bioprotectors. Aqueous solution of lead nitrate was injected into the stomach fasting at a dose of 36 mg/kg of body weight. Pectin, calcium, and antioxidants – C, E, β-carotene vitamins and selenium (Triovit® capsule) were used as biological protectors against the negative effects of lead. Drinking water was administered to the animals of the control groups, and appropriately selected bioprotectors were added to the diet. The conjugated dienes, malondialdehyde, superoxide dismutase activity, catalase activity, and antioxidant activity index were determined in the blood and liver tissue. An integral coefficient characterizing the antioxidant state of the body was calculated based on the ratios of the antioxidant protection indicator activity and the intensity of lipid peroxidation processes.
Results. It was found that during the experiment the level of lipid peroxidation products was increased: conjugated dienes by 70.8-114.8% in the blood, by 27.3-94.6% in the liver, malondialdehyde by 28.3-97.6% and 24.5-91.5%, respectively, compared to the controls. At the same time, on day 15 of the experiment, the activity of superoxide dismutase increased by 34.2% in the blood, the enzyme activity practically did not change in the liver tissue, and the catalase activity decreased. At the end of the experiment, the activity of superoxide dismutase and catalase enzymes decreased, to a greater extent in blood than in liver tissue. The obtained results indicate an increase in oxidative stress in the dynamics of lead intoxication, which is confirmed by low values of integral coefficients. The addition of bioprotector complex to the diet throughout the experiment with the simultaneous lead intake restores balance in the system of lipid peroxidation – antioxidant protection of liver and blood tissue.
Conclusions. Lead nitrate has induced oxidative stress in experimental animals. The complex of bioprotectors – pectin, calcium, C, E, and β-carotene vitamins, and selenium, activates metabolic processes and optimally corrects the disturbance in the activity ratio of processes in the pro- and antioxidant system caused by the action of lead.
KEYWORDS:
Lead nitrate, blood, liver tissue, conjugated dienes, malondialdehyde, superoxide dismutase, catalase, bioprotectors.
REFERENCES:
1. Trakhtenberg IM, Dmytrukha NM, Lugovskyi SP, Chekman IS, Kuprii VO, Doroshenko AM. [Lead is a dangerous pollutant. The old and new problem]. Ukrainian Journal of Modern Toxicological Aspects. 2015;3։14-24. Ukrainian. Available from: http://protox.medved.kiev.ua/index.php/ua/issues/2015/3/item/450-lead-is-a-dangerous-pollutant-the-old-and-new-problem
2. [Essays on the toxicology of heavy metals]. I.1: Lead. Trakhtenberg IM., editor. Kyiv: Avicenna; 2016:107. Ukrainian.
3. Environmental Health Criteria. 165. Inorganic Lead. Geneva: WHO, 1995. 251 ð. Available from: https://apps.who.int/iris/bitstream/handle/10665/37241/9241571659-eng.pdf
4. Exposure to lead: a major public health concern, 2nd edition. WHO; 2021. Available from: https://www.who.int/publications/i/item/9789240037632
5. Tkachyshyn VS. [Intoxications by lead and its inorganic compounds]. Emergency Medicine. 2021;17(4):6-12. Ukrainian. doi: https://doi.org/10.22141/2224-0586.17.4.2021.237721
6. Gzhegotsky MR, Fedorenko VI, Shtabsky BM. [Essays of prophylactic medicine]. Shtabsky BM, editor. Lviv: Medytsyna i pravo. 2008:400. Ukrainian.
7. Zerbino DD, Solomenchuk TM. [Lead: the damage of vascular system]. Ukrainian Medical Journal. 2002;2(28):79-83. Ukrainian. Available from: https://api.umj.com.ua/wp/wp-content/uploads/archive/28/pdf/791_ukr.pdf
8. Ostrovska SS, Shatorna VF, Slesarenko OG, Gerasymchuk PG, Topka EG, Alekseenko ZK, Lyulko IV, Kosse VA. [Impact of lead on reproductive health of men].Ukr. Ž. Med. Bìol. Sportu. 2021;6;4(32):193-8. Ukrainian. doi: https//doi.org/10.26693/jmbs06.04.193
9. Biletska EÌ, Onul NM, Antonova OV, Hlavatska VI, Zemliakova TD, Holovkova TA, Kalinicheva VV, Bezub OV. [Biochemical changes in the organism of children as predictors of microsaturnism]. Zapor´ozhye Medical Journal. 2020;22;2:200-5. Ukrainian. doi: https://doi.org/10.14739/2310-1210.2020.2.200597
10. Flora G, Gupta D, Tiwari A. Toxicity of lead: A review with recent updates. Interdiscip. Toxicol. 2012;5(2):47-58. doi: https://doi.org/10.2478/v10102-012-0009-2
11. Mason LH, Harp JP, Han DY. Pb neurotoxicity: neuropsychological effects of leadtoxicity. Biomed Res Int. 2014:840547. doi: https://doi.org/10.1155/2014/840547
12. Ebrahimi M, Khalili N, Razi S, Keshavarz-Fathi M, Khalili N, Rezaei N. Effects of lead and cadmium on the immune system and cancer progression J. Environ Health Sci Eng. 2020;18:335-43. doi: https://doi.org/10.1007/s40201-020-00455-2
13. Balali-Mood M, Naseri K, Tahergorabi Z, Khazdair, MR, Sadeghi M. Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front. Pharmacol. 2021;12:1-19. doi: https://doi.org/10.3389/fphar.2021.643972
14. Reznikov OH, Polumbryk ÎÌ, Balion YH, Polumbryk MÎ. [Pro- and antioxidant systems and pathological processes in humans]. Visn. Nac. Akad. Nauk Ukr. 2014;10:7-29. Ukrainian. doi: https://doi.org/10.15407/visn2014.10.017
15. Korolenko TK. [Lead and its effect on the antioxidant system]. In: Aktualni Problemy Profilaktychnoi Medytsyny: Zb. Nauk. Prats. Lviv; 2002;5:31-3. Ukrainian.
16. Pershyn OI. [Oxidative stress in the pathogenesis of lied acetate influence]. World of Medicine and Biology. 2014;43(1):143-6. Ukrainian. Available from: https://docs.google.com/viewerng/viewer?url=https://womab.com.ua/upload/10.1/SMB-2014-01-142.pdf&embedded=true
17. Babiker F, Al-Kouh A, Kilarkaje N. Lead exposure induces oxidative stress, apoptosis, and attenuates protection of cardiac myocytes against ischemiareperfusion injury. Drug Chem Toxicol. 2019;42(2):147-56. doi: https://doi.org/10.1080/01480545.2018.1429460
18. Gholami M, Harchegani AB, Saeedian S, Owrang M, Parvizi MR. Effect of N-acetyl cysteine on oxidative stress and Bax and Bcl2 expression in the kidney tissue of rats exposed to lead. Ukr. Biochem. J. 2021;93(1):59-67. doi: https://doi.org/10.15407/ubj93.01.059
19. Bogden JD, Oleske JM, Louria DB. Lead poisoning – one approach to a problem that won’t go away. Enviroment. Health Persp. 1999;107(6):431-6. doi: https://doi.org/10.1289/ehp.105-1470406
20. [Preclinical studies of medicinal products: methodical recommendations]. Stefanov OV, editor. Kyiv: Avicenna, 2001. 527 p. Ukrainian.
21. Melnyk NA. [Indicators of lipid peroxidation and condition of antioxidant defense system in rats´ liver and kidneys with impact of different sized nanopartiicles PbS on the skin]. Experimental and Clinical Medicine. 2017;74(1):22-30. Ukrainian. Available from: https://ecm.knmu.edu.ua/article/view/540/519
22. Melnyk AV, Piliponova VV, Ahafonov KM. [Effect of omega-3 polyunsaturated fatty acids on oxidative stress markers in the brain of rats with lead intoxication]. Pharmacology and Drug Toxicology. 2022;16(5):331-7. Ukrainian. Available from: https://pharmtox-j.org.ua/index.php/pharmtox-j/article/view/190/187
23. Belenichev IF, Levitsky EL, Gubsky UI, Kovalenko SI, Marchenko OM. [Antioxidant system of protection organism (review)]. Ukrainian Journal of Modern Toxicological Aspects. 2002;3։24-31. Ukrainian. Available from: http://medved.kiev.ua/arhiv_mg/st_2002/02_3_3.htm
FOR CITATION:
Fedorenko YuV. [Processes of lipid peroxidation and antioxidant protection in blood and liver under lead exposure and their correction in experimental studies]. Hygiene of Populated Places. 2023;73:59-67. Ukrainian.