Hygiene of populated placesISSN: 2707-0441 eISSN: 2707-045X
Issue 71, 2021   -   Pages: 111-117
INVESTIGATION OF ISOLATED AND COMBINED ACTION OF CHLOROFORM AND ALUMINUM SULFATE WITH DRINKING WATER ON THE ERYTHROCYTE SYSTEM OF ANIMAL BLOOD
L.A. Tomashevskaya1, V.A. Prokopov1, T.Ye. Kravchun1, E.B. Lipovetskaya1, N.V. Didyk1
1 STATE INSTITUTION O.M. MARZIEIEV INSTITUTE FOR PUBLIC HEALTH NAMSU

: 614.777:628.166:546.121
https://doi.org/10.32402/hygiene2021.71.111

SUMMRY:
Objective: study of the peculiarities of the reactions of the erythrocyte blood system and eval-uation of the possibility of their use to detect functional shifts in the body of animals under the influ-ence of chloroform and aluminum sulfate.
Materials and methods: combined effect of chloroform and aluminum sulfate on erythrocyte parameters.
Results. The tendency to decrease the level of hemoglobin, decrease the content of hemoglo-bin in one erythrocyte and decrease the absolute number of erythrocytes may indicate a restructuring in the erythrocyte blood system. The detected changes may indicate different rates of synthesis and accumulation of hemoglobin in the erythrocyte cells of the bone marrow of experimental and control animals and the functional insufficiency of mature forms of erythrocytes in experimental animals. Functional insufficiency of oxygen transport function of erythrocytes can cause oxygen deficiency in the body, with the subsequent development of dysfunction of all organs and systems of the body.
Conclusions. Hematological studies revealed qualitative and quantitative changes in erythrocyte cells. It was found that the nature and severity of the effects depended on the active substance, their MPC levels (dose) and the time of their exposure. The most pronounced changes can be observed in groups of animals with the maximum level of load - 3 and 5 MPC, both in the isolated action of chloroform and A1, and under conditions of their combined action.

KEYWORDS:
chloroform, aluminum sulfate, combined action, erythrocyte blood system.

REFERENCES:
1. Yatsyk A.V. Vodohospodarska ekolohiia [Water Management Ecology]. Kyiv : Genesa ; 2004 ; 3 : 172-183 (in Ukrainian).
2. Prokopov V.O. Pytna voda Ukrainy: medyko-ekolohichni ta sanitarno-hihiienichni aspekty (za red. A.M. Serdiuka) [Drinking Water of Ukraine: Medico-Ecological and Sanitary-Hygienic Aspects. A.M. Serdyuk (ed.)]. Kyiv : VSV "Medicine" ; 2016 : 173174, 190196 (in Ukrainian).
3. Prokopov V.O., Chichkovska G.V., Zorina O.V. Khlororhanichni spoluky u pytnii vodi: faktory ta umovy yikh utvorennia [Chloroorganic Composites in the Drinking Water: Factor and Mindset]. Dovkillia i zdorovia [Environment and Health]. 2004 ; 2 (29) : 70-73 (in Ukrainian).
4. Schencka K.M., Sivaganesana M., Rice G.E. Correlations of Water Quality Parameters with Mutagenicity of Chlorinated Drinking Water Samples. Journal of Toxicology and Environmental Health. 2009 ; 72 (7) : 461-467. DOI : https://doi.org/10.1080/15287390802608940
5. Richardson S.D., Plewa M.J., Wagner E.D. et al. Occurrence, genotoxicity, and carcinogenicity of regulated and emerging disinfection by-products in drinking water: a review and roadmap for research. Mutation Research. 2007 ; 636 : 178-242. DOI : https://doi.org/10.1016/j.mrrev.2007.09.001
6. Zapolsky A.K. Pidhotovka vysokoiakisnoi vody v kharchovii promyslovosti [Preparation of High-Quality Water in the Food Industry]. IV Mizhnarodnyi Vodnyi Forum Akva Ukraina 2006. Mizhnarodnyi Forum Ekolohichni tekhnolohii 2006 [IV International Water Forum Aqua - Ukraine 2006. International Forum Ecological Technologies 2006]. 2006 : 338 339 (in Ukrainian).
7. Yaroshenko K.K., Shabanov M.V. Efektyvnist koahuliatsiinoho ochyshchennia vodnykh stokiv keramichnoho vyrobnytstva [The efficiency of coagulation treatment of water effluents of ceramic production]. In : Zbirnyk naukovykh prats Instytutu heokhimii navkolyshnoho seredovyshcha [Collection of scientific works of the Institute of Environmental Geochemistry]. Kyiv : IGNS ; 2011 ; 19 : 95-101 (in Ukrainian).
8. Andrusishina I.M. Aliuminii u pytnii vodi ta zdorovia liudyny [Aluminium in drinking water and healthy people]. Kyiv ; 2018 : 38 p. (in Ukrainian).
9. Shtabskiy B.M., Hzhehotskiy M.R. Xenobiotics, chemical homeostasis and human security. Lviv : Nautilus ; 1999 : 308 p.
10. Belkin A.D., Michurina S.V., Shulgyna A.V., Arkhipov S.A. et al. Effect of magnetic field and constantly industrial frequency lighting on blood rat. Hygiene and sanitation. 2005 ; 5 : 37-40.
11. Principles and methods for assessing direct immunotoxicity associated with exposure to chemicals. Geneva : WHO ; 1996 : 390 p.
12. Pishak V.P., Vysotska V.G., Magallas V.M. et al. Laboratorni tvaryny v medyko-bilohichnykh eksperymentakh [Laboratory Animals in Medical and Biological Experiments]. Chernivtsi : Medical University ; 2006 : 350 p. (in Ukrainian).
13. Antomonov M.Yu. Matematicheskaya obrabotka i analiz mediko-biologicheskikh dannykh [Mathematical Processing and Analysis of Biomedical Data]. Kyiv ; 2018 : 579 p. (in Russian).

FOR CITATION:
Tomashevskaya L.A., Prokopov V.A., Kravchun T.Ye., Lipovetskaya E.B., Didyk N.V. Doslidzhennia izolovanoi ta kombinovanoi dii khloroformu ta sulfatu aliuminiiu z pytnoiu vodoiu na erytrotsytarnu systemu krovi tvaryn [Investigation of Isolated and Combined Action of Chloroform and Aluminum Sulfate with Drinking Water on the Erythrocyte System of Animal Blood]. In : Hihiiena naselenykh mists : zb. nauk. pr. [Hygiene of Populated Places]. Kyiv ; 2021 ; 71 : 111-117 (in Ukrainian).