| Hygiene of populated places | ISSN: 2707-0441 eISSN: 2707-045X |
Issue 69, 2019 - Pages: 96-105
ASSESSMENT OF BIOCIDAL ACTION OF THE COMPOSITES OF SILVER NANOPARTICLES AND ANTIMICROBIALS
O.V. Surmasheva1, L.I. Romanenko1, N.O. Nikonova1, H.I. Korchak1, L.D. Kisterska1, O.B. Loginova1, V.P. Sadokhin1
1 STATE INSTITUTION “O.M. MARZIEIEV INSTITUTE FOR PUBLIC HEALTH” NAMSU
ÓÄÊ: 615.33:678.046.3
SUMMÀRY:
A search for new drugs to overcome the resistance of microorganisms and increase of the ef-fectiveness of antimicrobial therapy is an urgent problem in the prevention and treatment of infec-tious-and-inflammatory diseases. Silver, in the form of nanoparticles, allows us to decrease hundreds times the concentration of metal at the conservation of biocidal properties.
The purpose of the work is to evaluate the biocidal action of silver nanoparticles and antimi-crobial agents under their combined use. The antimicrobial activity of composites, based on a con-centrated colloidal solution of silver nanoparticles in glycerol ("Silver Shield-1000") and antimicro-bials of various chemical groups: streptomycin and kanamycin, cefazolin azithromycin and ofloxacin, was studied. We used microbiological methods: suspension test according to the European standards and method of batch dilution in nutrient medium.
The results of the application of nanosilver solution in the combination with antibiotics of the groups of aminoglycosides and cephalosporin indicate the excess of the cumulative effect of the com-ponents in comparison with the isolated effect of their components, a phenomenon of synergism was observed, which allowed to reduce the concentrations of the composite components at the preserva-tion of the bactericidal properties.
The purpose of the work is to evaluate the biocidal action of silver nanoparticles and antimi-crobial agents under their combined use. The antimicrobial activity of composites, based on a con-centrated colloidal solution of silver nanoparticles in glycerol ("Silver Shield-1000") and antimicro-bials of various chemical groups: streptomycin and kanamycin, cefazolin azithromycin and ofloxacin, was studied. We used microbiological methods: suspension test according to the European standards and method of batch dilution in nutrient medium.
The results of the application of nanosilver solution in the combination with antibiotics of the groups of aminoglycosides and cephalosporin indicate the excess of the cumulative effect of the com-ponents in comparison with the isolated effect of their components, a phenomenon of synergism was observed, which allowed to reduce the concentrations of the composite components at the preserva-tion of the bactericidal properties.
KEYWORDS:
antimicrobial action, silver nanoparticles, antimicrobials, combined effect.
REFERENCES:
1. Halawani E. M. Int. J. Curr. Microbiol. App. Sci. 2016 ; 5 (2) : 397-414. DOI : https://doi.org/10.20546/ijcmas.2016.502.045
2. Masycheva V.I., Danilenko Å.D., Belkina À.Î. et al. Remedium. 2008 ; 9 : 12-23 (in Russian).
3. Biletska Å.Ì., Chekman ².S., Onul N.Ì. et al. Medychni perspektyvy. 2013 ; 2 : 114-119 (in Ukrainian).
4. Lara H.H., Ayala-Nunes N.V., Ixtepan-Turrent L., Rodriguez-Padilla C. J. Nanobiotechnology. 2010 ; 8 : 1-10. DOI : https://doi.org/10.1186/1477-3155-8-1
5. Chekman ².Ñ., Mariievskyi V.F., Rybalko S.L., Davtian L.L., Didikin H.H. et al. Ukrainskyi medychnyi chasopys. 2015 ; 5 : 45-48 (in Ukrainian).
6. Eremenko À.Ì., Smirnova N.P., Mukha Yu.P. and Yashan G.P. Teoreticheskaya I eksperimentalnaya khimiya. 2010 ; 46 (2) : 67–86 (in Russian). DOI : https://doi.org/10.1007/s11237-010-9122-5
7. Sadokhin V.V., Loginova Î.B. and Kisterskaya L.D. Perspektivy nauki (Science prospects). 2013 ; 11(50) : 104-107 (in Russian).
8. Movchan B.À. Visnyk farmakolohii I farmatsii. 2007 ; 12 : 5-13 (in Russian).
9. Dudko D.A., Sadokhin V.P., Kisterski L. Patent UA 80513 C2 Odnostadiinyi sposib pryhotu-vannia vysokokontsentrovanykh suspenzii nanorozmirnykh chastok elektroprovidnykh materialiv na osnovi vodorozchynnykh i vodo nerozchynnykh ridyn ta prystrii dlia yoho zdiisnennia [One-Step Method for the Preparation of High-Concentrated Suspensions of Nanosize Particles of Conductive Materials Based on Water-Soluble and Water-Insoluble Liquids and Device for its Implementation]. Publ. 25.09.2007; Bull. ¹15, 2007 (in Ukrainian).
10. DSTU EN 1276:2019 Sredstva khimicheskie dezinfitsiruiushchie i antiseptiki. Kolichestvennyi suspenzionnyi metod otsenky dlia opredeleniia bakteritsidnoy aktivnosti khimicheskikh dezinfitsiruiushchikh sredstv i antiseptikov, ispolzuemykh v uchrezhdeniyakh pitaniya, promyshlen-nosty, domashnem khoziaystve i obshchestvennykh zavedeniyakh. Metod ispytaniya i trebovaniya (etap 2, shag 1) (EN 1276:2009, IDT). S popravkoi ¹ 1:2019. [SSTC EN 1276: 2019 Chemical Disinfectants and Antiseptics. Quantitative Suspension Evaluation Method for the Determination of the Bactericidal Activity of Chemical Disinfectants and Antiseptics Used in Food, Industry, Household and Public Institutions. Test Method and Requirements (Stage 2, Step 1) (EN 1276: 2009, IDT). Amendment No. 1: 2019 ] (in Russian).
11. Derzhavnyi farmakolohichnyi tsentr MOZ Ukrainy. Vyvchennia spetsyfichnoi aktyvnosti protymikrobnykh likarskykh zasobiv: metodychni rekomendatsii [The Study of the Specific Activity of Antimicrobial Drugs: Guidelines]. Kyiv ; 2004 : 38 p. (in Ukrainian).
12. Birger M.O. (ed.). Spravochnyk po mykrobyolohycheskym y vyrusolohycheskym metodam yssledovanyia [Handbook of Microbiological and Virological Research Methods]. Moscow : Meditsina ; 1982 : 172-180 (in Russian).
FOR CITATION:
Surmasheva O.V., Romanenko L.I., Nikonova N.O., Korchak H.I., Kisterska L.D., Loginova O.B., Sadokhin V.P. Otsinka biotsydnoi dii kompozytiv z nanochastok sribla ta protymikrobnykh preparativ [Assessment of biocidal action of the composites of silver nanoparticles and antimicrobials]. In : Hihiiena naselenykh mists : zb. nauk. pr. [Hygiene of Populated Places]. Kyiv ; 2019 ; 69 : 96-105 (in Ukrainian).