Hygiene of populated placesISSN: 2707-0441 eISSN: 2707-045X
Issue 71, 2021   -   Pages: 117-125
MICROBIOLOGICAL METHODS FOR TOOTHPASTES QUALITY CONTROL IN UKRAINE
Z.. Oliinyk1, O.V. Surmasheva1, Yu.V. Zheludenko1
1 STATE INSTITUTION O.M. MARZIEIEV INSTITUTE FOR PUBLIC HEALTH NAMSU

: 613.49:579.63
https://doi.org/10.32402/hygiene2021.71.117

SUMMRY:
Objective. Conduct microbiological testing of toothpaste samples with verification of tech-niques to confirm the ability to detect microorganisms in the presence of the toothpaste.
Materials and methods. Test samples of toothpaste with active carbon content were tested according to GOST 7983-99. A number of mediums, test microorganisms and principles of methodological verification were used in accordance with State Pharmacopoeia of Ukraine.
Results. The microorganisms growth in the negative controls was not observed, in all positive controls a typical growth of used museum test strains was observed - study results were valid. Obtained results of samples microbiology testing satisfy the State Sanitary Rules and Regulations 2.2.9.027-99 requirements. According to the methods verification results it was found that the methods used to determine P. aeruginosa and Enterobacteriaceae were qualified while QMAFAnM, S. aureus, yeast and molds determination methods were considered unsuitable (the microorganisms test strains growth in the toothpaste sample presence was absent, indicating the antimicrobial activity). It has been proved that transfer to solid medium should be done regardless of the growth signs in the enrichment medium. The toothpastes antimicrobial activity may be lead to false-negative results of microbiology testing.
Conclusions. Studies have shown the microbiological parameters determining methods imperfection in the interstate standard GOST 7983-99 Toothpastes. General technical specifications. It is recommended to further perform toothpaste samples microbiology testing using the antimicrobial action neutralization method verified for each sample and invariably reseeding from enrichment medium to solid medium.

KEYWORDS:
toothpaste, microbial attributes, methods, method verification.

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FOR CITATION:
Oliinyk Z.., Surmasheva O.V., Zheludenko Yu.V. Metody kontroliu yakosti zubnykh past za mikrobiolohichnymy pokaznykamy v Ukraini [Microbiological Methods for Toothpastes Quality Control in Ukraine]. In : Hihiiena naselenykh mists : zb. nauk. pr. [Hygiene of Populated Places]. Kyiv ; 2021 ; 71 : 117-125 (in Ukrainian).