| Hygiene of populated places | ISSN: 2707-0441 eISSN: 2707-045X |
Issue 72, 2022 - Pages: 17-22
EXPERIMENTAL JUSTIFICATION FOR THE HYGIENIC REGULATIONS OF THE BARDAP 26 (ACTIVE INGREDIENT – BIS(DECYL)(2-HYDROXYETHYL METHYLAZANIUM PROPANOATE) IN AIR
V.A. Turkinà1, B.P. Kuzminov1, E.V. Tretyakova2, N.F. Lukasevich1, H.V. Shchurko1
1 DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
2 STATE ENTERPRISE «UKRAINIAN RESEARCH INSTITUTE OF TRANSPORT MEDICINE OF THE MINISTRY OF HEALTH OF UKRAINE»
2 STATE ENTERPRISE «UKRAINIAN RESEARCH INSTITUTE OF TRANSPORT MEDICINE OF THE MINISTRY OF HEALTH OF UKRAINE»
ÓÄÊ: 614.71:546.131
SUMMÀRY:
Objective. Scientific substantiation of health-related regulations on permissible content of “Bardap 26” biocidal product (active substance – bis(decyl)(2-hydroxyethyl methylazonium propanoate) in ambient air.
Materials and methods. Technical concentrate of “Bardap 26” product with a content of 80% of the active substance in ethylene glycol, diethylene glycol and water. Study methods – sanitary-chemical, toxicological, and statistical.
Results. The results outlined of determining marker volatile component of the product. It was established that the dynamic equilibrium of all migrating components in the exposure chamber is recorded in 24 hours. Ethylene glycol (up to 30% of product content) does not migrate from the product at 20°C due to its high boiling point – 197.3°C. At the same time, studies of ammonia migration showed that 0.016 mg of ammonia releases from 1 g of “Bardap 26” under model conditions. Studies have been conducted of “Bardap 26” toxic effects on mammals’ body with different routes of administration. In terms of acute oral toxicity, the product ranks as Hazard Class 3. The value of the median lethal concentration (CL 50) in the conditions of product inhalation could not be reached. It has a moderate skin irritant effect and a significant irritant effect on the chorioallantoic membrane of chicken embryos. Product exerts significant cumulative properties. It does not cause sensitization of the body. In the conditions of a chronic inhalation study, “Bardap 26” product did not cause clinical signs of intoxication and mortality among animals under conditions of our experiment.
Conclusions. Given the pre-set toxicity parameters, no prerequisites for the formation of an aerosol of the product both in the process of manufacturing ready-made disinfectants and their application as well, the results of studying chemical composition of mixture of substances that migrate from “Bardap 26” product under model conditions, it is advisable to inspect ambient air based on the marker compound (ammonia) concentration.
Materials and methods. Technical concentrate of “Bardap 26” product with a content of 80% of the active substance in ethylene glycol, diethylene glycol and water. Study methods – sanitary-chemical, toxicological, and statistical.
Results. The results outlined of determining marker volatile component of the product. It was established that the dynamic equilibrium of all migrating components in the exposure chamber is recorded in 24 hours. Ethylene glycol (up to 30% of product content) does not migrate from the product at 20°C due to its high boiling point – 197.3°C. At the same time, studies of ammonia migration showed that 0.016 mg of ammonia releases from 1 g of “Bardap 26” under model conditions. Studies have been conducted of “Bardap 26” toxic effects on mammals’ body with different routes of administration. In terms of acute oral toxicity, the product ranks as Hazard Class 3. The value of the median lethal concentration (CL 50) in the conditions of product inhalation could not be reached. It has a moderate skin irritant effect and a significant irritant effect on the chorioallantoic membrane of chicken embryos. Product exerts significant cumulative properties. It does not cause sensitization of the body. In the conditions of a chronic inhalation study, “Bardap 26” product did not cause clinical signs of intoxication and mortality among animals under conditions of our experiment.
Conclusions. Given the pre-set toxicity parameters, no prerequisites for the formation of an aerosol of the product both in the process of manufacturing ready-made disinfectants and their application as well, the results of studying chemical composition of mixture of substances that migrate from “Bardap 26” product under model conditions, it is advisable to inspect ambient air based on the marker compound (ammonia) concentration.
KEYWORDS:
“Bardap 26” product (bis(decyl)(2-hydroxyethyl methylazonium propanoate), toxicity studies, mammals, health-related regulations.
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FOR CITATION:
Turkinà VA, Kuzminov BP, Tretyakova EV, Lukasevich NF, Shchurko HV. Experimental justification for the hygienic regulations of the bardap 26 (active ingredient – bis(decyl)(2-hydroxyethyl methylazanium propanoate) in air. Hygiene of populated places. 2022;72:17-22. Ukrainian.